With any iPSC lines, it is necessary to confirm pluripotency and characterize the cell lines before the following downstream regulation experiments. Teratoma formation is an essential criterion not only for determining the pluripotency but also for assessing the tumorigenic potential of human iPSCs. Now Creative Biolabs has built a standard protocol for teratoma assay based on subcutaneous co-transplantation of human iPSCs with mitotically inactivated feeder cells and matrix into immunodeficient mice.
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